Rate constants are determinable outside the original Michaelis–Menten mathematical formalism wherein the substrate concentration range is  1.6  4.8 times enzyme concentration: A pre-steady-state scenario and beyond

For some time now, there has been growing interest in pre-steady-state (PSS) kinetic parameters for whatever reasons, the measurement of which needs high-tech equipment capable transient time-scale duration assay. The proposition, however, is that all parameters, PSS and beyond, can be determined with appropriate derivable equations usual Michaelis-Menten (MM) Briggs-Haldane (BH) equations, respectively. objectives research were: 1) To derive determination reverse rate constant when substrate concentration, [S] « MM constant, KM, 2) determine by calculation, forward consequently, show it possible to often seen masked within original cum BH mathematical formalism, 3) validate corollaries from derivation justify procedural issue. Theoretical, experimental (Bernfeld method), computational methods were explored. Pre-steady-state k-1, process ES ® E + S, 2nd order k1, rate, v1, formation enzyme-substrate complex, ES, derived. derived originating associated validated have reproducing variables parameters; constants seemed formalism unmasked. Steady-state (SS) zero » their values. “Negative” catalytic efficiency (k-1/KM) was “positive” efficiency, (kcat/KM), lower [ET]. In conclusion, derivable. Previously MM/BB now calculated using data; thus, regardless reaction pathway's state, PSS, SS. SS/zero